Measuring motion on DNA by the type I restriction endonuclease EcoR124I using triplex displacement
The type I restriction enzyme EcoR124I cleaves DNA following extensive linear translocation dependent upon ATP hydrolysis. Using protein-directed displacement of a DNA triplex, we have determined the kinetics of one-dimensional motion without the necessity of measuring DNA or ATP hydrolysis. The tri...
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| Publicat a: | EMBO J |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Nature Publishing Group
2000
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC305691/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10790375/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/emboj/19.9.2094 |
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