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AMP-dependent DNA relaxation catalyzed by DNA ligase occurs by a nicking-closing mechanism.

In the presence of AMP and Mg2+, a covalently closed duplex DNA containing negative superhelical turns was treated with DNA ligase isolated from bacteriophage T4-infected E. coli. This resulted in the gradual and not sudden loss of superhelical turns as for example in the case of type I DNA topoisom...

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Main Authors: Montecucco, A, Ciarrocchi, G
格式: Artigo
語言:Inglês
出版: 1988
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC338414/
https://ncbi.nlm.nih.gov/pubmed/3137526
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