Functional characterization of the T4 DNA ligase: a new insight into the mechanism of action.

ATP-dependent DNA ligases are essential enzymes in both DNA replication and DNA repair processes. Here we report a functional characterization of the T4 DNA ligase. One N-terminal and two C-terminal deletion mutants were expressed in Escherichia coli as histidine- tagged proteins. An additional muta...

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Bibliographic Details
Main Authors: Rossi, R, Montecucco, A, Ciarrocchi, G, Biamonti, G
Format: Artigo
Language:Inglês
Published: 1997
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC146716/
https://ncbi.nlm.nih.gov/pubmed/9153309
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