Functional characterization of the T4 DNA ligase: a new insight into the mechanism of action.

ATP-dependent DNA ligases are essential enzymes in both DNA replication and DNA repair processes. Here we report a functional characterization of the T4 DNA ligase. One N-terminal and two C-terminal deletion mutants were expressed in Escherichia coli as histidine- tagged proteins. An additional muta...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Rossi, R, Montecucco, A, Ciarrocchi, G, Biamonti, G
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1997
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146716/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9153309/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.11.2106
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