AMP-dependent DNA relaxation catalyzed by DNA ligase occurs by a nicking-closing mechanism.
In the presence of AMP and Mg2+, a covalently closed duplex DNA containing negative superhelical turns was treated with DNA ligase isolated from bacteriophage T4-infected E. coli. This resulted in the gradual and not sudden loss of superhelical turns as for example in the case of type I DNA topoisom...
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| Veröffentlicht in: | Nucleic Acids Res |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1988
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC338414/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3137526/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.15.7369 |
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