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AMP-dependent DNA relaxation catalyzed by DNA ligase occurs by a nicking-closing mechanism.
In the presence of AMP and Mg2+, a covalently closed duplex DNA containing negative superhelical turns was treated with DNA ligase isolated from bacteriophage T4-infected E. coli. This resulted in the gradual and not sudden loss of superhelical turns as for example in the case of type I DNA topoisom...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1988
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC338414/ https://ncbi.nlm.nih.gov/pubmed/3137526 |
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