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Tuning the Drug Efflux Activity of an ABC Transporter in vivo by in vitro Selected DARPin Binders

ABC transporters use the energy from binding and hydrolysis of ATP to import or extrude substrates across the membrane. Using ribosome display, we raised designed ankyrin repeat proteins (DARPins) against detergent solubilized LmrCD, a heterodimeric multidrug ABC exporter from Lactococcus lactis. Se...

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書誌詳細
主要な著者: Seeger, Markus A., Mittal, Anshumali, Velamakanni, Saroj, Hohl, Michael, Schauer, Stefan, Salaa, Ihsene, Grütter, Markus G., van Veen, Hendrik W.
フォーマット: Artigo
言語:Inglês
出版事項: Public Library of Science 2012
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC3366976/
https://ncbi.nlm.nih.gov/pubmed/22675494
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0037845
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