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Tuning the Drug Efflux Activity of an ABC Transporter in vivo by in vitro Selected DARPin Binders
ABC transporters use the energy from binding and hydrolysis of ATP to import or extrude substrates across the membrane. Using ribosome display, we raised designed ankyrin repeat proteins (DARPins) against detergent solubilized LmrCD, a heterodimeric multidrug ABC exporter from Lactococcus lactis. Se...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3366976/ https://ncbi.nlm.nih.gov/pubmed/22675494 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0037845 |
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