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Folding and activity of mutant cystathionine β-synthase depends on the position and nature of the purification tag: Characterization of the R266K CBS mutant

Cystathionine beta-synthase (CBS), a heme-containing PLP-dependent enzyme, catalyzes the condensation of serine and homocysteine to yield cystathionine. Missense mutations in CBS, the most common cause of homocystinuria, often result in misfolded proteins. Arginine 266, where the pathogenic missense...

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Detalles Bibliográficos
Main Authors: Majtan, Tomas, Kraus, Jan P.
Formato: Artigo
Idioma:Inglês
Publicado: 2012
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3319291/
https://ncbi.nlm.nih.gov/pubmed/22333527
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2012.01.019
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