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High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase.
We demonstrate that despite lacking a 3'----5' proofreading exonuclease, the Thermus aquaticus (Taq) DNA polymerase can catalyze highly accurate DNA synthesis in vitro. Under defined reaction conditions, the error rate per nucleotide polymerized at 70 degrees C can be as low as 10(-5) for...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1990
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC331072/ https://ncbi.nlm.nih.gov/pubmed/2374708 |
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