DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.

The highly thermostable DNA polymerase from Thermus aquaticus (Taq) is ideal for both manual and automated DNA sequencing because it is fast, highly processive, has little or no 3'-exonuclease activity, and is active over a broad range of temperatures. Sequencing protocols are presented that produce...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Innis, M A, Myambo, K B, Gelfand, D H, Brow, M A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1988
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282767/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3200828/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.24.9436
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