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DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.

The highly thermostable DNA polymerase from Thermus aquaticus (Taq) is ideal for both manual and automated DNA sequencing because it is fast, highly processive, has little or no 3'-exonuclease activity, and is active over a broad range of temperatures. Sequencing protocols are presented that produce...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Innis, M A, Myambo, K B, Gelfand, D H, Brow, M A
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1988
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282767/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3200828/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.24.9436
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