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DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.
The highly thermostable DNA polymerase from Thermus aquaticus (Taq) is ideal for both manual and automated DNA sequencing because it is fast, highly processive, has little or no 3'-exonuclease activity, and is active over a broad range of temperatures. Sequencing protocols are presented that pr...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1988
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282767/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3200828/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.24.9436 |
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