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DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.

The highly thermostable DNA polymerase from Thermus aquaticus (Taq) is ideal for both manual and automated DNA sequencing because it is fast, highly processive, has little or no 3'-exonuclease activity, and is active over a broad range of temperatures. Sequencing protocols are presented that pr...

詳細記述

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書誌詳細
主要な著者: Innis, M A, Myambo, K B, Gelfand, D H, Brow, M A
フォーマット: Artigo
言語:Inglês
出版事項: 1988
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC282767/
https://ncbi.nlm.nih.gov/pubmed/3200828
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