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High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase.

We demonstrate that despite lacking a 3'----5' proofreading exonuclease, the Thermus aquaticus (Taq) DNA polymerase can catalyze highly accurate DNA synthesis in vitro. Under defined reaction conditions, the error rate per nucleotide polymerized at 70 degrees C can be as low as 10(-5) for...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteurs principaux: Eckert, K A, Kunkel, T A
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 1990
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC331072/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2374708/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.13.3739
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