High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase.
We demonstrate that despite lacking a 3'----5' proofreading exonuclease, the Thermus aquaticus (Taq) DNA polymerase can catalyze highly accurate DNA synthesis in vitro. Under defined reaction conditions, the error rate per nucleotide polymerized at 70 degrees C can be as low as 10(-5) for base subst...
Guardat en:
| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1990
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC331072/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2374708/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.13.3739 |
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