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Expression and purification of E. coli BirA biotin ligase for in vitro biotinylation
The extremely tight binding between biotin and avidin or streptavidin makes labeling proteins with biotin a useful tool for many applications. BirA is the Escherichia coli biotin ligase that site-specifically biotinylates a lysine side chain within a 15-amino acid acceptor peptide (also known as Avi...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2012
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3288220/ https://ncbi.nlm.nih.gov/pubmed/22227598 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2011.12.008 |
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