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Expression and purification of E. coli BirA biotin ligase for in vitro biotinylation

The extremely tight binding between biotin and avidin or streptavidin makes labeling proteins with biotin a useful tool for many applications. BirA is the Escherichia coli biotin ligase that site-specifically biotinylates a lysine side chain within a 15-amino acid acceptor peptide (also known as Avi...

Полное описание

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Библиографические подробности
Главные авторы: Li, Yifeng, Sousa, Rui
Формат: Artigo
Язык:Inglês
Опубликовано: 2012
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC3288220/
https://ncbi.nlm.nih.gov/pubmed/22227598
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2011.12.008
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