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Mammalian expression vectors for metabolic biotinylation tandem affinity tagging by co-expression in cis of a mammalian codon-optimized BirA biotin ligase
ΟBJECTIVE: To construct mammalian expression vectors for the N- or C-terminal tagging of proteins with a tandem affinity tag comprised of the biotinylatable Avi tag and of a triple FLAG tag. RESULTS: We constructed and tested by transient transfections mammalian expression vectors for the co-express...
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| Publicado no: | BMC Res Notes |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6001059/ https://ncbi.nlm.nih.gov/pubmed/29898783 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13104-018-3500-9 |
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