Lataa...
Isolation, cloning, and expression of E. coli BirA gene for biotinylation applications
BACKGROUND: The key enzyme in biotin-(strept) avidin systems, Escherichia coli BirA biotin ligase, is currently obtained by overexpression of the long protein-tagged versions of the gene to prevent its toxic effect in E. coli. Herein we describe a rather simple and efficient system for expression of...
Tallennettuna:
| Julkaisussa: | Adv Biomed Res |
|---|---|
| Päätekijät: | , , , , |
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
Medknow Publications & Media Pvt Ltd
2015
|
| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4551058/ https://ncbi.nlm.nih.gov/pubmed/26380234 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4103/2277-9175.161576 |
| Tagit: |
Lisää tagi
Ei tageja, Lisää ensimmäinen tagi!
|