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Isolation, cloning, and expression of E. coli BirA gene for biotinylation applications
BACKGROUND: The key enzyme in biotin-(strept) avidin systems, Escherichia coli BirA biotin ligase, is currently obtained by overexpression of the long protein-tagged versions of the gene to prevent its toxic effect in E. coli. Herein we describe a rather simple and efficient system for expression of...
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| Publicado no: | Adv Biomed Res |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Medknow Publications & Media Pvt Ltd
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4551058/ https://ncbi.nlm.nih.gov/pubmed/26380234 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4103/2277-9175.161576 |
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