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Site-specific biotinylation of purified proteins using BirA
The binding between biotin and streptavidin or avidin is one of the strongest known non-covalent biological interactions. The (strept)avidin-biotin interaction has been widely used for decades in biological research and biotechnology. Therefore labeling of purified proteins by biotin is a powerful w...
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| Publicado no: | Methods Mol Biol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4304673/ https://ncbi.nlm.nih.gov/pubmed/25560075 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2272-7_12 |
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