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Isolation of secreted proteins from Drosophila ovaries and embryos through in vivo BirA-mediated biotinylation
The extraordinarily strong non-covalent interaction between biotin and avidin (kD = 10(−14)–10(−16)) has permitted this interaction to be used in a wide variety of experimental contexts. The Biotin Acceptor Peptide (BAP), a 15 amino acid motif that can be biotinylated by the E. coli BirA protein, ha...
Enregistré dans:
| Publié dans: | PLoS One |
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| Auteurs principaux: | , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Public Library of Science
2019
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6816556/ https://ncbi.nlm.nih.gov/pubmed/31658274 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0219878 |
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