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Co-expression of BirA with biotin bait achieves in vivo biotinylation of overexpressed stable N-glycosylated sRAGE in transgenic silkworms
Here, we demonstrated the expression of the N-glycosylated extracellular ligand binding domain of receptor for advanced glycation end products (sRAGE) in middle silk glands (MSGs) of transgenic silkworms using the GAL4/UAS system. Over 1 mg of sRAGE was obtained from one transgenic silkworm. sRAGE p...
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Veröffentlicht in: | Sci Rep |
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Hauptverfasser: | , , , , , , |
Format: | Artigo |
Sprache: | Inglês |
Veröffentlicht: |
Nature Publishing Group UK
2017
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Schlagworte: | |
Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5428419/ https://ncbi.nlm.nih.gov/pubmed/28336960 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-00420-4 |
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