Co-expression of BirA with biotin bait achieves in vivo biotinylation of overexpressed stable N-glycosylated sRAGE in transgenic silkworms

Here, we demonstrated the expression of the N-glycosylated extracellular ligand binding domain of receptor for advanced glycation end products (sRAGE) in middle silk glands (MSGs) of transgenic silkworms using the GAL4/UAS system. Over 1 mg of sRAGE was obtained from one transgenic silkworm. sRAGE p...

Full description

Saved in:
Bibliographic Details
Published in:Sci Rep
Main Authors: Kumano-Kuramochi, Miyuki, Tatematsu, Ken-ichiro, Ohnishi-Kameyama, Mayumi, Maeda-Yamamoto, Mari, Kobori, Toshiro, Sezutsu, Hideki, Machida, Sachiko
Format: Artigo
Language:Inglês
Published: Nature Publishing Group UK 2017
Subjects:
Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5428419/
https://ncbi.nlm.nih.gov/pubmed/28336960
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-00420-4
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items