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A complex of methylthioadenosine/S-adenosylhomocysteine nucleosidase, transition state analogue and nucleophilic water identified by mass spectrometry
An enzyme-stabilized nucleophilic water molecule has been implicated at the transition state of Escherichia coli methylthioadenosine nucleosidase (EcMTAN) by transition state analysis and by crystallography. We analyzed the EcMTAN mass in complex with a femtomolar transition state analogue to determ...
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| Главные авторы: | , , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2012
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3268516/ https://ncbi.nlm.nih.gov/pubmed/22239413 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja211176q |
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