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A complex of methylthioadenosine/S-adenosylhomocysteine nucleosidase, transition state analogue and nucleophilic water identified by mass spectrometry

An enzyme-stabilized nucleophilic water molecule has been implicated at the transition state of Escherichia coli methylthioadenosine nucleosidase (EcMTAN) by transition state analysis and by crystallography. We analyzed the EcMTAN mass in complex with a femtomolar transition state analogue to determ...

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Main Authors: Wang, Shanzhi, Lim, Jihyeon, Thomas, Keisha, Yan, Funing, Angeletti, Ruth H., Schramm, Vern L.
Format: Artigo
Jezik:Inglês
Izdano: 2012
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3268516/
https://ncbi.nlm.nih.gov/pubmed/22239413
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja211176q
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