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Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA

In vitro-transcribed mRNA has great therapeutic potential to transiently express the encoded protein without the adverse effects of viral and DNA-based constructs. Mammalian cells, however, contain RNA sensors of the innate immune system that must be considered in the generation of therapeutic RNA....

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Bibliografski detalji
Glavni autori: Karikó, Katalin, Muramatsu, Hiromi, Ludwig, János, Weissman, Drew
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2011
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3241667/
https://ncbi.nlm.nih.gov/pubmed/21890902
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr695
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