Improvements to solid phase phosphotriester synthesis of deoxyoligonucleotides.

A solution of benzenesulphonic acid (3%, w/v) in a dimethylformamide and dichloromethane mixture (9:1, v/v) is shown to be a very effective reagent for the detritylation of deoxyoligonucleotides attached to a solid support. The levels of depurination with this reagent were lower than those observed...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Patel, T P, Millican, T A, Bose, C C, Titmas, R C, Mock, G A, Eaton, M A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1982
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320910/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7145710/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.18.5605
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