Improvements to solid phase phosphotriester synthesis of deoxyoligonucleotides.
A solution of benzenesulphonic acid (3%, w/v) in a dimethylformamide and dichloromethane mixture (9:1, v/v) is shown to be a very effective reagent for the detritylation of deoxyoligonucleotides attached to a solid support. The levels of depurination with this reagent were lower than those observed...
Sparad:
| I publikationen: | Nucleic Acids Res |
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| Huvudupphov: | , , , , , |
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Utgiven: |
Oxford University Press
1982
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| Länkar: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320910/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7145710/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.18.5605 |
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