Improvements to solid phase phosphotriester synthesis of deoxyoligonucleotides.

A solution of benzenesulphonic acid (3%, w/v) in a dimethylformamide and dichloromethane mixture (9:1, v/v) is shown to be a very effective reagent for the detritylation of deoxyoligonucleotides attached to a solid support. The levels of depurination with this reagent were lower than those observed...

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Bibliographic Details
Main Authors: Patel, T P, Millican, T A, Bose, C C, Titmas, R C, Mock, G A, Eaton, M A
Format: Artigo
Language:Inglês
Published: 1982
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC320910/
https://ncbi.nlm.nih.gov/pubmed/7145710
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