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Improvements to solid phase phosphotriester synthesis of deoxyoligonucleotides.
A solution of benzenesulphonic acid (3%, w/v) in a dimethylformamide and dichloromethane mixture (9:1, v/v) is shown to be a very effective reagent for the detritylation of deoxyoligonucleotides attached to a solid support. The levels of depurination with this reagent were lower than those observed...
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| Hauptverfasser: | , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1982
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC320910/ https://ncbi.nlm.nih.gov/pubmed/7145710 |
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