Polymer supported DNA synthesis using hydroxybenzotriazole activated phosphotriester intermediates.

Phosphorylation of N-acyl-5'-O-DMTr-d-nucleosides, or similarly protected DNA-dimers having a free 3'-OH group, with 2-chlorophenyl-0,0-bis(1-benzotriazolyl)phosphate affords reactive 3'-phosphotriester derivatives. The latter intermediates can be used, without further purification, for the synthesi...

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Detalles Bibliográficos

Publicado en:	Nucleic Acids Res
Principais autores:	Marugg, J E, Piel, N, McLaughlin, L W, Tromp, M, Veeneman, G H, van der Marel, G A, van Boom, J H
Formato:	Artigo
Idioma:	Inglês
Publicado:	Oxford University Press 1984
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320404/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6095205/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.22.8639
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