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Polymer supported DNA synthesis using hydroxybenzotriazole activated phosphotriester intermediates.
Phosphorylation of N-acyl-5'-O-DMTr-d-nucleosides, or similarly protected DNA-dimers having a free 3'-OH group, with 2-chlorophenyl-0,0-bis(1-benzotriazolyl)phosphate affords reactive 3'-phosphotriester derivatives. The latter intermediates can be used, without further purification, f...
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| Autors principals: | , , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
1984
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| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC320404/ https://ncbi.nlm.nih.gov/pubmed/6095205 |
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