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A general method for the synthesis of 5'-monophosphates of DNA fragments via phosphotriester intermediates.
A new and attractive phosphorylation procedure which allows the introduction, via phosphotriester intermediates, of 5'-phosphate functions of DNA fragments is described. The method is based on the activation of bifunctional phosphorylating agents with 1-hydroxybenzotriazole. The approach will b...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1982
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC320613/ https://ncbi.nlm.nih.gov/pubmed/7088712 |
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