A general method for the synthesis of 5'-monophosphates of DNA fragments via phosphotriester intermediates.

A new and attractive phosphorylation procedure which allows the introduction, via phosphotriester intermediates, of 5'-phosphate functions of DNA fragments is described. The method is based on the activation of bifunctional phosphorylating agents with 1-hydroxybenzotriazole. The approach will be exe...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	van der Marel, G A, van Boeckel, C A, Wille, G, van Boom, J H
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1982
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320613/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7088712/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.7.2337
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