A general method for the synthesis of 5'-monophosphates of DNA fragments via phosphotriester intermediates.
A new and attractive phosphorylation procedure which allows the introduction, via phosphotriester intermediates, of 5'-phosphate functions of DNA fragments is described. The method is based on the activation of bifunctional phosphorylating agents with 1-hydroxybenzotriazole. The approach will be exe...
Gardado en:
| Publicado en: | Nucleic Acids Res |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
1982
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| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320613/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7088712/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.7.2337 |
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