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A general method for the synthesis of 5'-monophosphates of DNA fragments via phosphotriester intermediates.

A new and attractive phosphorylation procedure which allows the introduction, via phosphotriester intermediates, of 5'-phosphate functions of DNA fragments is described. The method is based on the activation of bifunctional phosphorylating agents with 1-hydroxybenzotriazole. The approach will b...

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: van der Marel, G A, van Boeckel, C A, Wille, G, van Boom, J H
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 1982
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC320613/
https://ncbi.nlm.nih.gov/pubmed/7088712
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