Polymer supported DNA synthesis using hydroxybenzotriazole activated phosphotriester intermediates.
Phosphorylation of N-acyl-5'-O-DMTr-d-nucleosides, or similarly protected DNA-dimers having a free 3'-OH group, with 2-chlorophenyl-0,0-bis(1-benzotriazolyl)phosphate affords reactive 3'-phosphotriester derivatives. The latter intermediates can be used, without further purification, for the synthesi...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1984
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320404/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6095205/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.22.8639 |
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