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Polymer supported DNA synthesis using hydroxybenzotriazole activated phosphotriester intermediates.

Phosphorylation of N-acyl-5'-O-DMTr-d-nucleosides, or similarly protected DNA-dimers having a free 3'-OH group, with 2-chlorophenyl-0,0-bis(1-benzotriazolyl)phosphate affords reactive 3'-phosphotriester derivatives. The latter intermediates can be used, without further purification, for the synthesi...

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Gorde:
Argitaratua izan da:Nucleic Acids Res
Egile Nagusiak: Marugg, J E, Piel, N, McLaughlin, L W, Tromp, M, Veeneman, G H, van der Marel, G A, van Boom, J H
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Oxford University Press 1984
Sarrera elektronikoa:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320404/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6095205/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.22.8639
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