A rapid deprotection procedure for phosphotriester DNA synthesis.

An equimolar solution of aldoxime and tetramethylguanidine at 70 degrees C removes both the base and phosphate protection from oligonucleotides prepared by solid phase phosphate triester technology. The rate of cleavage from the succinyl linkage commonly used for solid phase synthesis is also increa...

Полное описание

Библиографические подробности

Опубликовано в::	Nucleic Acids Res
Главные авторы:	Patel, T P, Chauncey, M A, Millican, T A, Bose, C C, Eaton, M A
Формат:	Artigo
Язык:	Inglês
Опубликовано:	Oxford University Press 1984
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320121/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6483622/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.17.6853
Метки:	Добавить метку Нет меток, Требуется 1-ая метка записи!