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A rapid deprotection procedure for phosphotriester DNA synthesis.

An equimolar solution of aldoxime and tetramethylguanidine at 70 degrees C removes both the base and phosphate protection from oligonucleotides prepared by solid phase phosphate triester technology. The rate of cleavage from the succinyl linkage commonly used for solid phase synthesis is also increa...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Nucleic Acids Res
Prif Awduron: Patel, T P, Chauncey, M A, Millican, T A, Bose, C C, Eaton, M A
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 1984
Mynediad Ar-lein:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320121/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6483622/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.17.6853
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