A rapid deprotection procedure for phosphotriester DNA synthesis.
An equimolar solution of aldoxime and tetramethylguanidine at 70 degrees C removes both the base and phosphate protection from oligonucleotides prepared by solid phase phosphate triester technology. The rate of cleavage from the succinyl linkage commonly used for solid phase synthesis is also increa...
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| Publicado en: | Nucleic Acids Res |
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| Autores principales: | , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
1984
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| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320121/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6483622/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.17.6853 |
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