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A rapid deprotection procedure for phosphotriester DNA synthesis.

An equimolar solution of aldoxime and tetramethylguanidine at 70 degrees C removes both the base and phosphate protection from oligonucleotides prepared by solid phase phosphate triester technology. The rate of cleavage from the succinyl linkage commonly used for solid phase synthesis is also increa...

詳細記述

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書誌詳細
出版年:Nucleic Acids Res
主要な著者: Patel, T P, Chauncey, M A, Millican, T A, Bose, C C, Eaton, M A
フォーマット: Artigo
言語:Inglês
出版事項: Oxford University Press 1984
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320121/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6483622/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.17.6853
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