Pyrovanadolysis, a Pyrophosphorolysis-like Reaction Mediated by Pyrovanadate, Mn(2+), and DNA Polymerase of Bacteriophage T7

DNA polymerases catalyze the 3′–5′-pyrophosphorolysis of a DNA primer annealed to a DNA template in the presence of pyrophosphate (PP(i)). In this reversal of the polymerization reaction, deoxynucleotides in DNA are converted to deoxynucleoside 5′-triphosphates. Based on the charge, size, and geomet...

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Hauptverfasser: Akabayov, Barak, Kulczyk, Arkadiusz W., Akabayov, Sabine R., Theile, Christopher, McLaughlin, Larry W., Beauchamp, Benjamin, van Oijen, Antoine M., Richardson, Charles C.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Biochemistry and Molecular Biology 2011
Schlagworte:
Enzymology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3190722/
https://ncbi.nlm.nih.gov/pubmed/21697085
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.250944
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