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Direct measurement of the protein response to an electrostatic perturbation that mimics the catalytic cycle in ketosteroid isomerase

Understanding how electric fields and their fluctuations in the active site of enzymes affect efficient catalysis represents a critical objective of biochemical research. We have directly measured the dynamics of the electric field in the active site of a highly proficient enzyme, Δ(5)-3-ketosteroid...

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Bibliografiske detaljer
Main Authors: Jha, Santosh Kumar, Ji, Minbiao, Gaffney, Kelly J., Boxer, Steven G.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3189056/
https://ncbi.nlm.nih.gov/pubmed/21949360
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1113874108
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