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A Critical Test of the Electrostatic Contribution to Catalysis with Non-canonical Amino Acids in Ketosteroid Isomerase
The vibrational Stark effect (VSE) has been used to measure the electric field in the active site of ketosteroid isomerase (KSI). These measured fields correlate with ΔG(‡) in a series of conventional mutants yielding an estimate for the electrostatic contribution to catalysis (Fried et al. Science,...
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| Gepubliceerd in: | J Am Chem Soc |
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| Hoofdauteurs: | , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
2016
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5063566/ https://ncbi.nlm.nih.gov/pubmed/27545569 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.6b06843 |
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