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Extreme electric fields power catalysis in the active site of ketosteroid isomerase
Enzymes use protein architecture to impose specific electrostatic fields onto their bound substrates, but the magnitude and catalytic effect of these electric fields have proven difficult to quantify with standard experimental approaches. Using vibrational Stark effect spectroscopy, we found that th...
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| Publicado no: | Science |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4668018/ https://ncbi.nlm.nih.gov/pubmed/25525245 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.1259802 |
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