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A Preorganized Electric Field Leads to Minimal Geometrical Reorientation in the Catalytic Reaction of Ketosteroid Isomerase

Electrostatic interactions play a pivotal role in enzymatic catalysis and are increasingly modeled explicitly in computational enzyme design; nevertheless, they are challenging to measure experimentally. Using vibrational Stark effect (VSE) spectroscopy, we have measured electric fields inside the a...

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Detalhes bibliográficos
Publicado no:J Am Chem Soc
Main Authors: Wu, Yufan, Fried, Stephen D., Boxer, Steven G.
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7474534/
https://ncbi.nlm.nih.gov/pubmed/32378409
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.0c00383
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