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A Preorganized Electric Field Leads to Minimal Geometrical Reorientation in the Catalytic Reaction of Ketosteroid Isomerase

Electrostatic interactions play a pivotal role in enzymatic catalysis and are increasingly modeled explicitly in computational enzyme design; nevertheless, they are challenging to measure experimentally. Using vibrational Stark effect (VSE) spectroscopy, we have measured electric fields inside the a...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:J Am Chem Soc
Päätekijät: Wu, Yufan, Fried, Stephen D., Boxer, Steven G.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2020
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC7474534/
https://ncbi.nlm.nih.gov/pubmed/32378409
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.0c00383
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