Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish

Zinc-finger nucleases (ZFNs) allow targeted gene inactivation in a wide range of model organisms. However, construction of target-specific ZFNs is technically challenging. Here, we evaluate a straightforward modular assembly-based approach for ZFN construction and gene inactivation in zebrafish. Fro...

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Main Authors: Zhu, Cong, Smith, Tom, McNulty, Joseph, Rayla, Amy L., Lakshmanan, Abirami, Siekmann, Arndt F., Buffardi, Matthew, Meng, Xiangdong, Shin, Jimann, Padmanabhan, Arun, Cifuentes, Daniel, Giraldez, Antonio J., Look, A. Thomas, Epstein, Jonathan A., Lawson, Nathan D., Wolfe, Scot A.
格式: Artigo
語言:Inglês
出版: Company of Biologists 2011
主題:
Technical Paper
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3177320/
https://ncbi.nlm.nih.gov/pubmed/21937602
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dev.066779
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