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An optimized two-finger archive for ZFN-mediated gene targeting

The widespread use of zinc finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the r...

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Autori principali: Gupta, Ankit, Christensen, Ryan G., Rayla, Amy L., Lakshmanan, Abirami, Stormo, Gary D., Wolfe, Scot A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3443678/
https://ncbi.nlm.nih.gov/pubmed/22543349
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1994
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