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A modified bacterial one-hybrid system yields improved quantitative models of transcription factor specificity

We examine the use of high-throughput sequencing on binding sites recovered using a bacterial one-hybrid (B1H) system and find that improved models of transcription factor (TF) binding specificity can be obtained compared to standard methods of sequencing a small subset of the selected clones. We ca...

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Detalhes bibliográficos
Main Authors: Christensen, Ryan G., Gupta, Ankit, Zuo, Zheng, Schriefer, Lawrence A., Wolfe, Scot A., Stormo, Gary D.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2011
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3130293/
https://ncbi.nlm.nih.gov/pubmed/21507886
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr239
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