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Combining SELEX with quantitative assays to rapidly obtain accurate models of protein–DNA interactions
Models for the specificity of DNA-binding transcription factors are often based on small amounts of qualitative data and therefore have limited accuracy. In this study we demonstrate a simple and efficient method of affinity chromatography-SELEX followed by a quantitative binding (QuMFRA) assay to r...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1236725/ https://ncbi.nlm.nih.gov/pubmed/16186128 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni139 |
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