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Combining SELEX with quantitative assays to rapidly obtain accurate models of protein–DNA interactions

Models for the specificity of DNA-binding transcription factors are often based on small amounts of qualitative data and therefore have limited accuracy. In this study we demonstrate a simple and efficient method of affinity chromatography-SELEX followed by a quantitative binding (QuMFRA) assay to r...

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Bibliografske podrobnosti
Main Authors: Liu, Jiajian, Stormo, Gary D.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2005
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1236725/
https://ncbi.nlm.nih.gov/pubmed/16186128
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni139
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